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實驗使用(yòng)用(yòng)谷氨酸棒狀杆菌WT菌株ATCC13032。實驗裝(zhuāng)置（如圖 1）有(yǒu) Lucullus（Securecell，瑞士）和EVE生物(wù)工(gōng)藝平台設備（瑞士，Infors）作(zuò)為(wèi)工(gōng)藝監控和記錄的信息管理(lǐ)系統。
To facilitate optimal process control limiting substrates must be identified, moni-tored and controlled. Amino acids are often critical substances, especially in mam-malian processes. Measurement and thus monitoring is tedious and rarely done.
HPLC is the common reference method for product and metabolite analysis in bio-processes. Sampling is needed for these analyses, resulting in off-line measure-ments along the process. In order to reduce time and cost expenses in process development a
